Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.
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A rapid and accurate diagnosis of PRV infection is important for the initiation of appropriate control strategies. The assay proved to be very sensitive due to as little as 1. Author information Article notes Copyright and License information Disclaimer. The virus principally affects pigs, which are considered to be the natural host for PRV and the reservoir of the virus in nature, but also infects a broad range of wild and non-porcine mammals with the important exception of higher-order primates 8.
PCR experiments were performed on serial ten-fold dilutions of a viral suspension of PRV isolate V with a titer of 10 6. The analytical sensitivity of the test was estimated to be 1. Primers sequences, genome positions and the size of PCR products are shown in Table 1.
Negative controls were run with each test.
Doença de Aujeszky – Wikipédia, a enciclopédia livre
Traditionally, PRV detection is based on direct virus isolation followed by confirmation using immunofluorescence, immunoperoxidase or neutralization tests with specific antiserum 2. National Center for Biotechnology InformationU.
Articles from Brazilian Journal of Microbiology are provided here courtesy of Soena.
Oligonucleotide primers and restriction endonuclease selection PRV specific primers were designed using the Oligo 6. Since the rapid detection of infected animals would reduce the potential transmission of the viruses to uninfected herds avoiding the spread of the diseases The trigeminal ganglion is the most consistent site for virus isolation, although latent virus is usually difficult to culture or even impossible 113 and PCR is the method recommended to detect viral genome present in this site.
Open in a separate window. Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and in pigs with emphasis on the respiratory tract. The isolation and characterization of a strain of infectious bovine rhinotracheitis virus from stillbirth in swine. In general, PRV infections must be considered in the differential diagnosis of respiratory, reproductive and nervous disorders.
Non-specific reactions were not observed when a related herpesvirus, other porcine DNA genome viruses and uninfected cells were used to assess PCR. Diagnostic methods for detection of Classical swine fever virus—Status quo and new developments. Also, the BLAST search against nucleotide databases of different herpesvirus and random nucleotide sequences revealed this region is very specific for PRV genomes.
The polymerase chain reaction PCR is a rapid tool that can be used no only to detect acutely PRV infected pigs but it is the recommended test for detect PRV latent infection. The conventional diagnostic procedure is time-consuming and false-negative results may occur in submissions e, latently infected animals.
Doença de Aujeszky
Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome. A rapid, sensitive and specific PCR-based diagnostic assay to detect pseudorabies virus in clinical samples is provided.
Cell biological and molecular characteristics of pseudorabies virus infections in cell cultures and pigs with emphasis on respiratory tract. The virus primarily replicates in the respiratory tract, spreads along cranial nerves to the brains and via lymph and blood to internal organs, with the reproductive organs being affected. Potential sites of virus latency associated with indigenous pseudorabies viruses in feral swine. Table 1 Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome.
This gene codes for an envelope glycoprotein named gD which plays and important role in binding cellular receptors and is critical for virus replication in different organs The viral agent following a primary replication can establish latent infection and develops a latency-reactivation infection which allows its perpetuation in pig populations 1012 Especially HVB1 is an important target for specificity assay because is a related herpesvirus which is known to infect swine BHV-1 4.
The development, optimization and evaluation of a polymerase chain reaction PCR assay are presented for the diagnosis of pseudorabies infection. Multiplex PCR for the simultaneous detection of pseudorabies virus, porcine cytomegalovirus, and porcine circovirus in pigs.
Each one of the nine tissue field samples from pigs diagnosed as PRV infected based on clinical signs and laboratory aujeszkt yielded the corresponding PRV amplified product when analyzed. Finally, nucleic acids from tissue homogenates samples derived from seven healthy pigs, and a non infected PK cell line were also tested showing no positive products data not shown.