Cultivo y fusion de protoplastos . Loyola Vargas, V.M. Rosell, C.H. (ed.) Villalobos Arambula, V.M. (ed.) Access the full text: NOT AVAILABLE. Lookup the . Un protoplasto es una célula de planta, bacteria u hongo que ha perdido total o parcialmente su pared celular, para lo cual se usan. Index terms: Citrus sp., gamma irradiation, grapefruit, protoplast fusion, somatic foi de protoplastos de ‘Ruby Red’ irradiados com 80 Gy, fusionados com.
|Published (Last):||20 August 2007|
|PDF File Size:||13.72 Mb|
|ePub File Size:||18.61 Mb|
|Price:||Free* [*Free Regsitration Required]|
Potato, Solanum tuberosum L. Protoplasts were precipitated by centrifugation at rpm during 10 minutes. RAPD and chromosomal analysis of the hybrids are also presented. Aggregations of more than two protoplasts were also observed. Protoplast fusion between haploid gametic and diploid somatic cells has been achieved which clearly demonstrate that fusion is independent of cell type.
Results and discussion Protoplasts fusion and plant regeneration Protoplasts fusion from S. These results showed that all hybrids were aneuploids Fig. Nineteen somatic hybrid plants were obtained and confirmed by RAPD analysis. Production of somatic hybrids between frost – tolerant Solanum commersonii and Solanum tuberosum: Both 6carboxyfluorescein and the kanamycin resistance harboured by the plasmid have been transferred from liposomes to protoplasts of phycomyces by the fusion technique.
Fusion de protoplastos pdf
The production of somatic hybrid plants with a prltoplastos number deviating from the expected is well documented in many fusion combinations where potato protoplasts have been used as one or both fusion parents Austin et al. Interspecific somatic hybrids between wild potato Solanum acaule Bitt. Isolation of mesophyll protoplasts from leaves of dalbergia. Using chemical or electrical procedures Jones, protoplasts from different donor plants can be fused together and somatic hybrids regenerated from the fusion products.
This technique is a tool for somatic hybrid protoplastow Baird et al.
When colonies developed into microcalli, the alginate beads were depolimerized. Ruiz de Galarreta y E.
Fusion de Protoplastos by Arely Gutierrez on Prezi
Non-transgenic applications of plant tissue culture in potato. Gazala nasim were incubated in different concentrations of mannitol and combinations of enzyme solutions.
After that, colonies were dispersed into plates with solid MS13K regeneration medium Benke, Regeneration in Gewebekulturen einiger Dihaploider Solanum tuberosum Pgotoplastos. This study shows that somatic hybrid potato plants can be obtained by the fusion method presented.
Cultivo y fusion de protoplastos 
After seven days, the culture medium was diluted 1: Solanum, protoplast fusion, polyethylene glycol, somatic hybrid, RAPD analysis. The wild species S. Characterization of somatic hybrids of potato by use of RAPD markers and isozyme analysis. A rapid calli proliferation was observed and most of them showed purple-colored cells Fig. Regeneration of isolated protoplasts to plants in Solanum dulcamara. The osmolality of this medium was fixed at mOsm. Tissue culture procedures to produce somatic hybrids and the application of molecular biological tools for their analysis are well established.
The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article.
None of the regenerants had 72 chromosomes, which results from the protoplasts fusion of a tetraploid with a diploid genome. Production of somatic hybrids by electrofusion in Solanum. Universidad Nacional Agraria La Molina. It has been demonstrated that protoplast-derived plants show a higher degree of chromosomal number variation than those derived from tissue or organ culture. The RAPD analysis was carried out for all regenerated plants derived from calli of the combination S.
Then, at fifty days, all culture media were replaced by a mOsm V-KM medium. Regenerants from the fusion experiments were propagated in vitro using the Murashige and Skoog medium.
The effect of initial culture media. After protoplasts fusion and during plant regeneration it frequently happens that genetic changes such as variations in chromosome number occur.
Protoplasts of both types are mixed in an equal proportion attaining a density of 5 x 10 4 to 2 x 10 5 protoplasts per ml. These plants were multiplied in glass culture tubes for further analysis.
Chromosome analysis The numbers of chromosome of S. Nineteen plants were regenerated from calli Fig.
This value is different from that obtained by Mattheij et al. An analysis of putative fusion products is essential to confirm hybrid status Masuelli et al.
New York, NY Acknowledgments The authors would like to thank the International Potato Center for financial support and to Martha Williams de Castro who revised the English version of this article.
The ploidy of the genotype used in plant regeneration has been shown to profoundly affect the type of variation observed in morphological characters and chromosome numbers among regenerant plants.
Somatic hybrids were confirmed morphologically under greenhouse conditions, when intermediate characteristics between parent lines, such as shoot coloration, leaf shape and protoplasyos were observed. Protoplasts were precipitated by centrifugation at rpm during five minutes. Analysis of chromatin, nuclear DNA and organelle composition in somatic hybrids between Solanum tuberosum and Solanum sanctae-rosae.
Shoot regeneration started twelve weeks after calli were transferred to MS medium.